Recombinant Human 4-1BBL/TNFSF9 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P41273
- 应用性： Cell culture
| 别称 |
Tumor necrosis factor ligand superfamily member 9;4-1BB ligand;4-1BBL;TNFSF9
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| 表达系统 |
E.coli
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| 序列 |
Arg71-Asp 140
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| 蛋白编码 |
P41273
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| 种属 |
Human
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| 计算分子量 |
20.4 kDa
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| 表观分子量 |
20 kDa
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| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is 1-5 ng/mL.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain; placenta; lung; skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells; and is thought to be involved in T cell-tumor cell interaction.
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实验操作视频
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