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  • 抗体及相关试剂
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  • 细胞分离与鉴定
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Recombinant Human AQP-3 protein (GST tag)

Recombinant Human AQP-3 protein (GST tag)
  • Recombinant Human AQP-3 protein (GST tag)
  • Recombinant Human AQP-3 protein (GST tag)

Price: ¥826.00 ¥476.00

Size:
100 μg 20 μg
  • 表达系统: E.coli
  • 蛋白编码: Q92482
别称
AQP 3;AQP-3;AQP3;Aqp3;Aquaglyceroporin-3;Aquaporin 3;Aquaporin-3;Aquaporin3;GIL;Gill blood group
表达系统
E.coli
序列
Leu 110-Ser 170
蛋白编码
Q92482
种属
Human
计算分子量
31.6 kDa
表观分子量
37 kDa
标签
N-GST
生物活性
Not validated for activity
纯度
> 95% as determined by reducing SDS-PAGE.
内毒素
Please contact us for more information.
保存条件
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件
This product is provided as lyophilized powder which is shipped with ice packs.
制剂
Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
复溶方法
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景
Water channel required to promote glycerol permeability and water transport across cell membranes.Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
> 95 % as determined by reducing SDS-PAGE.

Q1:无标签蛋白纯化方式?

蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。

Q2:重组蛋白有活性吗?可以做活性检测吗?

活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。

Q3:蛋白Buffer里是否含有denaturants(变性剂)?

我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。

Q4:蛋白可以用于细胞培养/注射小鼠吗?

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q5:蛋白的运输条件是什么?

我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。

Q6:蛋白是通过什么方式纯化的?

一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q7:蛋白发货形式可以是冻干粉吗?可以发液体吗?

默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。



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