Recombinant Human ATG10 Protein (His&T7 Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9H0Y0
| 别称 |
Ubiquitin-Like-Conjugating Enzyme ATG10;Autophagy-Related Protein 10;APG10-Like;ATG10
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| 表达系统 |
E.coli
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| 序列 |
Met 1-Thr190
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| 蛋白编码 |
Q9H0Y0
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| 种属 |
Human
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| 计算分子量 |
24.5 kDa
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| 表观分子量 |
30 kDa
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| 标签 |
N-T7 & C-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 20% Glycerol, 0.02% Tween 80, 3mM TCEP, pH 8.5.
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| 复溶方法 |
Not Applicable
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| 背景 |
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.
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实验操作视频
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