Recombinant Human BCAS2/DAM1 Protein (His & T7 Tag)
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- 表达系统： E.coli
- 蛋白编码： O75934
| 别称 |
Pre-mRNA-Splicing Factor SPF27;Breast Carcinoma-Amplified Sequence 2;DNA Amplified in Mammary Carcinoma 1 Protein;Spliceosome-Associated Protein SPF 27;BCAS2;DAM1
|
| 表达系统 |
E.coli
|
| 序列 |
Ala2-Phe225
|
| 蛋白编码 |
O75934
|
| 种属 |
Human
|
| 计算分子量 |
28.6 kDa
|
| 表观分子量 |
60 kDa
|
| 标签 |
N-T7 & C-His
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 2mM DTT, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.
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实验操作视频
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