Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P00915
| 别称 |
Carbonic Anhydrase 1;Carbonate Dehydratase I;Carbonic Anhydrase B;CAB;Carbonic Anhydrase I;CA-I;CA1
|
| 表达系统 |
E.coli
|
| 序列 |
Ala2-Phe261
|
| 蛋白编码 |
P00915
|
| 种属 |
Human
|
| 计算分子量 |
29.9 kDa
|
| 表观分子量 |
25-35 kDa
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| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 12.5mM Tris-HCl, 75mM NaCl, pH 7.5.
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| 复溶方法 |
Not Applicable
|
| 背景 |
Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.
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实验操作视频
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