Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： AAH05279.1
| 别称 |
Carboxypeptidase A1;CPA1;CPA
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Lys17-Tyr419
|
| 蛋白编码 |
AAH05279.1
|
| 种属 |
Human
|
| 计算分子量 |
46.6 kDa
|
| 表观分子量 |
44 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mm NaCl, pH 7.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.
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实验操作视频
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