Recombinant Human eIF4E protein (GST,His tag)
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- 表达系统： E.coli
- 蛋白编码： P06730
| 别称 |
Eukaryotic translation initiation factor 4E;eIF-4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein;EIF4E;EIF4EL1;EIF4F
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| 表达系统 |
E.coli
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| 序列 |
Ala 2-Val 217
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| 蛋白编码 |
P06730
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| 种属 |
Human
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| 计算分子量 |
48.7 kDa
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| 表观分子量 |
52 kDa
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| 标签 |
N-GST & C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
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实验操作视频
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