Recombinant Human HMGB1 Protein
Price:
- 表达系统: E.coli
- 蛋白编码: P09429
别称 |
High Mobility Group Protein B1;High Mobility Group Protein 1;HMG-1;HMGB1;HMG1
|
表达系统 |
E.coli
|
序列 |
Met1-Phe89
|
蛋白编码 |
P09429
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种属 |
Human
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计算分子量 |
10.4 kDa
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表观分子量 |
14 kDa
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标签 |
None
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 500mM NaCl, 0.5mMDTT, pH 7.9.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.
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实验操作视频
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