Recombinant Human Interleukin-3/IL-3 Protein (Human Cells, His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P08700
- 应用性： Cell culture
| 别称 |
Interleukin-3;IL-3;Hematopoietic Growth Factor;Mast Cell Growth Factor;MCGF;Multipotential Colony-Stimulating Factor;P-Cell-Stimulating Factor;IL3
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| 表达系统 |
HEK293 Cells
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| 序列 |
Ala20-Phe152
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| 蛋白编码 |
P08700
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| 种属 |
Human
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| 计算分子量 |
16.1 kDa
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| 表观分子量 |
17-30 kDa
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| 标签 |
C-His
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| 生物活性 |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.3-1. 5 ng/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity; and is thought to be associated with neurologic disorders.
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实验操作视频
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