Recombinant Human MASP1 protein (His tag)
Price:
- 表达系统： E.coli
- 蛋白编码： P48740
| 别称 |
Mannan-Binding Lectin Serine Protease 1;Complement Factor MASP-3;Mannose-Binding Lectin-Associated Serine Protease 1;MASP-1;RaRF;Serine Protease 5;MASP1;CRARF;CRARF1;PRSS5
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| 表达系统 |
E.coli
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| 序列 |
His 20-Ala 447
|
| 蛋白编码 |
P48740
|
| 种属 |
Human
|
| 计算分子量 |
47.0 kDa
|
| 表观分子量 |
48 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Mannan-Binding Lectin Serine Protease 1 (MASP-1) belongs to the peptidase S1 family. MASP1 contains two CUB domains, one EGF-like domain, one peptidase S1 domain and two Sushi (CCP/SCR) domains. MASP1 is primarily expressed in liver. MASP1 involved in the lectin pathway of the complement, performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. MASP1 is synthesized as a zymogen and activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. MASP1 is not directly involved in complement activation but may act as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP2. MASP1 is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. MASP1 is inhibited by SERPING1 and A2M.
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Q1:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q2:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
实验操作视频
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