Recombinant Human RSPO3 Protein (Fc & His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9BXY4
| 别称 |
R-spondin-3;RSPO3;Protein with TSP type-1 repeat;Roof plate-specific spondin-3;Thrombospondin type-1 domain-containing protein 2;PWTSR;THSD2;CRISTIN1
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| 表达系统 |
HEK293 Cells
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| 序列 |
Gln22-Val201
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| 蛋白编码 |
Q9BXY4
|
| 种属 |
Human
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| 计算分子量 |
47.9 kDa
|
| 表观分子量 |
61 kDa
|
| 标签 |
C-Fc-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
R-spondin-3 (RSPO3); also known as Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2 and CRISTIN1; is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases; and one TSP type-1 domain; RSPO3 founctions as a activator of the beta-catenin signaling cascade; leading to TCF-dependent gene activation. Otherwise; RSPO3 may negatively regulate the TGF-beta pathway.
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实验操作视频
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