Recombinant Human S100A13 Protein
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- 表达系统: E.coli
- 蛋白编码: Q99584
别称 |
Protein S100-A13;S100A13;S100 calcium-binding protein A13
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表达系统 |
E.coli
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序列 |
Ala2-Lys98
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蛋白编码 |
Q99584
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种属 |
Human
|
计算分子量 |
11.3 kDa
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表观分子量 |
12-15 kDa
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标签 |
None
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 1mM CaCl2, 0.1% Tween-20, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
S100A13 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. It is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. It plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. It binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium binding. It is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine.
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实验操作视频
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