Recombinant Human SELENOI/EPT1 Protein (GST Tag)
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- 表达系统: E.coli
- 蛋白编码: Q9C0D9
别称 |
Ethanolaminephosphotransferase 1;hEPT1;Selenoprotein I;SelI;EPT1;KIAA1724;SELI
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表达系统 |
E.coli
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序列 |
Met 1-Pro50
|
蛋白编码 |
Q9C0D9
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种属 |
Human
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计算分子量 |
32.6 kDa
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表观分子量 |
29 kDa
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标签 |
N-GST
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.0.
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复溶方法 |
Not Applicable
|
背景 |
Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway.
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实验操作视频
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