Recombinant Human Zic3 protein (His tag)
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- 表达系统： E.coli
- 蛋白编码： O60481
| 别称 |
Zinc finger protein ZIC 3;ZIC3;Zinc finger protein 203;Zinc finger protein of the cerebellum 3
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| 表达系统 |
E.coli
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| 序列 |
Met 1-Val467
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| 蛋白编码 |
O60481
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| 种属 |
Human
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| 计算分子量 |
51.3 kDa
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| 表观分子量 |
52 kDa
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| 标签 |
N-His & C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
Please contact us for more information.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N?terminal poly-Ala (aa 46?55) and poly-His (aa 87?97) segments, followed by five C2H2-type Zn-fingers (aa 253?410). Within the zinc finger region, there is an overlapping NES (aa 290?349) and two NLS's (aa 367?382 and 403?410). ZIC3 reportedly binds to GLI-3.
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实验操作视频
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