Recombinant Klebsiella pneumoniae NEO Protein
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- 表达系统： E.coli
- 蛋白编码： P00552
| 别称 |
Aminoglycoside 3'-phosphotransferase;APH(3')-II;APH(3')II;Kanamycin kinase type II;Neomycin-kanamycin phosphotransferase type II;neo
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| 表达系统 |
E.coli
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| 序列 |
Met1-Phe264
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| 蛋白编码 |
P00552
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| 种属 |
Klebsiella pneumoniae
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| 计算分子量 |
29 kDa
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| 表观分子量 |
26-30 kDa
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| 标签 |
None
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween80, PH8.0.
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| 复溶方法 |
Not Applicable
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| 背景 |
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.
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实验操作视频
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