Recombinant Mouse EGF Protein (His Tag)
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- 表达系统: E.coli
- 蛋白编码: P01132
- 应用性: Cell culture
别称 |
Pro-epidermal growth factor;Epidermal growth factor;EGF
|
表达系统 |
E.coli
|
序列 |
Asn977-Arg1029
|
蛋白编码 |
P01132
|
种属 |
Mouse
|
计算分子量 |
7.0 kDa
|
表观分子量 |
8 kDa
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标签 |
C-His
|
生物活性 |
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <80 pg/mL. The specific activity of recombinant mouse EGF is approximately >1 x 107 IU/mg.
|
纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
EGF is a single-pass type I membrane protein,containing 8 LDL-receptor class B repeats and 9 EGF-like domains. EGF results in cellular proliferation, differentiation, and survival.EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
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实验操作视频
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