Recombinant Mouse HGF Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q08048
| 别称 |
Hepatopoietin-A;Scatter factor;SF;Deafnes;Autosomal recessive 39;DFNB39;F-TCF;Hepatocyte growth factor
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| 表达系统 |
HEK293 Cells
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| 序列 |
Gln33-Leu728
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| 蛋白编码 |
Q08048
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| 种属 |
Mouse
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| 计算分子量 |
80.0 kDa
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| 表观分子量 |
60 kDa
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| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions.
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实验操作视频
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