Recombinant Mouse IFN alpha 1a protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P01572
- 应用性： Cell culture
| 别称 |
FIL1 delta;IL-1F5;IL-1HY1;IL-1L1;IL-1RP3;IL-1ra Homolog 1;IL-1 delta
|
| 表达系统 |
E.coli
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| 序列 |
Cys 24-Lys 189
|
| 蛋白编码 |
P01572
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| 种属 |
Mouse
|
| 计算分子量 |
19.9 kDa
|
| 表观分子量 |
17-25 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 x 107 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes, and they also increase the ability of uninfected host cells to resist new infection by the virus. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.
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实验操作视频
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