Recombinant Rat Adrenomedullin/ADM protein (His tag)
Price:
- 表达系统： E.coli
- 蛋白编码： P43145
| 别称 |
ADM precursor;Adrenomedullin;PAMP;Preproadrenomedullin;ProAM N terminal 20 peptide;ProAM N20
|
| 表达系统 |
E.coli
|
| 序列 |
Thr 21-Leu 185
|
| 蛋白编码 |
P43145
|
| 种属 |
Rat
|
| 计算分子量 |
18.0 kDa
|
| 表观分子量 |
25 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Adrenomedullin (ADM; also AM) is a secreted, monomeric, 6 kDa member of the Adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. ADM has multiple functions, including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The ADM preproprecursor is 185 amino acids (aa) in length. It contains a 21 aa signal sequence, a processed 20 aa peptide termed PAMP (aa 22-41), an N-terminal propeptide (aa 45-91), the ADM precursor (amidation is required for maturation) (aa 94 143), and a C-terminal propeptide (aa 150-185). The ADM precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated ADM (aa 94 143).
|
Q1:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q2:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
实验操作视频
新品推荐
Recombinant Human IL-6 Protein (Fc Tag)
Recombinant Mouse BCR protein (His tag)
Recombinant Mouse IL6 protein(His tag)
Recombinant Rat IL2/IL-2/Interleukin-2 protein (His tag)
Recombinant SARS-CoV-2 Nucleocapsid Protein (His Tag)
Recombinant Human Fyn protein(His tag)
Recombinant Mouse CD96/TACTILE protein (His tag)
