Recombinant Vaccinia virus Soluble Interferon α/β Receptor B18 Protein (His Tag)
Price:
Size:
50μg
10μg
- 表达系统: HEK293 Cells
- 蛋白编码: P25213
别称 |
Soluble interferon alpha/beta receptor B18;VACWR200
|
表达系统 |
HEK293 Cells
|
序列 |
His20-Glu351
|
蛋白编码 |
P25213
|
种属 |
Vaccinia virus
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计算分子量 |
39.2 kDa
|
表观分子量 |
50-65 kDa
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标签 |
C-His
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
B18R, also known as B19R in the Copenhagen strain of Vaccinia, is a type I interferon (IFN)-binding protein encoded by the B18R open reading frame in the Western Reserve strain of vaccinia virus. B18R exists in two forms, soluble and membrane-bound form and also has a broad species specificity. B18R has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. Secreted B18R binds to uninfected and infected cells. B18R presents at the cell surface and protects cells from the antiviral state. It has shown that binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.
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实验操作视频
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