TBP Polyclonal Antibody
Price:
Size:
500μL
300μL
120μL
60μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB
Verified Samples |
Verified Samples in WB: Rat brain, Mouse intestine, Mouse lung,HepG2 |
Dilution | WB 1:4000-1:6000 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Synthetic peptide of Human TATA-box-binding protein |
Abbre | TBP |
Synonyms | GTF2D; GTF2D1; HDL4; SCA17; TATA binding factor; TATA box binding protein; TATA box factor; TATA sequence binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; TATA-box-binding protein; TBP; TBP; TFIID; Transcription initiation factor TFIID TBP subunit抗体 |
Swissprot | |
Calculated MW | 38 kDa |
Observed MW | 38 kDa |
Cellular Localization | Nucleus |
Tissue Specificity | Widely expressed, with levels highest in the testis and ovary. |
Concentration | 1mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Background | The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP,along with a variety of TBP-associated factors,make up the TFIID,a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner,it helps position RNA polymerase II over the transcription start site of the gene. However,it is estimated that only 10-20% of human promoters have TATA boxes. Therefore,TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa). |
Western Blot analysis of Rat brain, Mouse intestine, Mouse lung and HepG2 cells using TBP Polyclonal Antibody at dilution of 1:5000.
E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
E-AB-1025 | Goat Anti-Rabbit IgG(H+L)(Biotin conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |