Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
中文名称:总超氧化物歧化酶(T-SOD)比色法测试盒(WST-1 法)
Price:
Size:
96T(92 samples)
48T(44 samples)
- 样本类型: 血清(浆);胸水;腹水;尿液;细胞;动植物组织
- 检测仪器及波长: 酶标仪(440-460 nm)
- 研究领域: 氧化应激,植物生理系列,铁死亡
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
WST-1法的原理参考下图,WST-1可以和黄嘌呤氧化酶(Xanthine Oxidase,XO)催化产生的超氧化物阴离子(O2.-)反应产生水溶性的甲臜染料(formazan dye),由于SOD能催化超氧化物阴离子发生歧化反应,所以该反应步骤可以被SOD所抑制,因此SOD的活性与甲臜染料的生成量呈负相关,通过对WST-1产物的比色分析即可计算SOD的活力。
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指标简称 | T-SOD |
灵敏度 | 0.2 U/mL |
检测范围 | 0.2 -14.4 U/mL |
精密度 | 批间差:3.7% | 批内差:2.9% |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(440-460 nm) |
保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存12个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 12个月 |
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