XRCC4 Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human
- 应用性： WB;IHC-p;IF;IP
| Verified Samples |
Verified Samples in WB:Hela,293T Verified Samples in IHC:Human breast cancer Verified Samples in IF:Human liver cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-300;IF 1:50-1:200;IP 1:100-1:300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Abbre | XRCC4 |
| Synonyms | DNA double strand break repair and V(D)J recombination protein XRCC4;DNA repair protein XRCC4;SSMED;X ray repair complementing defective repair in Chinese hamster cells 4;X ray repair cross complementing 4;X ray repair cross complementing protein 4;X-ray repair cross-complementing protein 4;XRCC 4;XRCC4;XRCC4抗体 |
| Swissprot | |
| Calculated MW | 38kDa |
| Observed MW | 38kDa |
| Cellular Localization | Nucleus. |
| Tissue Specificity | Widely expressed. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling |
| Clone No. | Clone:8K2 |
| Conjugation | Unconjugated |
| Background | Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
Western Blot analysis of 1) Hela, 2) 293T cells using XRCC4 Monoclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using XRCC4 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human liver cancer tissue using XRCC4 Monoclonal Antibody at dilution of 1:200.
