RIPA Lysis Buffer (Strong)
中文名称:RIPA裂解液 (强)
Price:
Size:
100mL
50mL
20mL
- 应用性: WB
产品简介 | RIPA裂解液是一种传统的细胞组织快速裂解液,作为Western Blot试验中从动物组织或细胞中提取蛋白的首选裂解液。 |
保存条件 | -20°C冻存,保质期12个月。 |
注意事项 | 1. 裂解样品的所有步骤都需在冰上或4°C进行。 2. PMSF和原矾酸钠在使用前添加,每1 mL裂解液中加入10 μL PMSF和10 μL Na3VO4,使终浓度为1 mM。如发现RIPA有沉淀,请放置室温或温水浴进行溶解。 3. RIPA裂解液的裂解产物中可能会出现一团透明胶状物,属正常现象。该透明胶状物为含有基因组DNA等的复合物。用移液器反复吹打样本50次左右,可以有效降低样本样本粘度,离心后取上清进行后续试验。 4. 用RIPA裂解的得到的蛋白样品,因含有高浓度的去垢剂,不能用Bradford法测定样品的蛋白浓度,建议使用BCA法测定蛋白浓度。 5. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 |
-
Oxaliplatin induces ferroptosis and oxidative stress in HT29 colorectal cancer cells by inhibiting the Nrf2 signaling pathway
IF: 2.447 -
Huanglian‑Houpo drug combination ameliorates H1N1‑induced mouse pneumonia via cytokines, antioxidant factors and TLR/MyD88/NF‑κB signaling pathways
IF: 2.447Journal:Experimental and Therapeutic Medicine -
Effect of Neiyi Prescription of QIU on autophagy and angiogenic ability of endometriosis via the PPARγ/NF-κB signaling pathway
IF: 2.344 -
Silencing of circular RNA circPDE5A suppresses neuroblastoma progression by targeting the miR-362-5p/NOL4L axis
IF: 2.292 -
CircRASSF2 facilitates the proliferation and metastasis of colorectal cancer by mediating the activity of Wnt/β-catenin signaling pathway by regulating the miR-195-5p/FZD4 axis
IF: 2.248 -
Protective effect of ischaemic postconditioning combined with nicorandil on myocardial ischaemia‒reperfusion injury in diabetic rats
IF: 2.174Journal:BMC Cardiovascular Disorders -
miR-486-5p Restrains Extracellular Matrix Production and Oxidative Damage in Human Trabecular Meshwork Cells by Targeting TGF-β/SMAD2 Pathway
IF: 1.909Journal:Journal of Ophthalmology -
Parkin enhances sensitivity of paclitaxel to NPC by arresting cell cycle
IF: 1.794 -
Effect of DEC1 on the proliferation, adhesion, invasion and epithelial‑mesenchymal transition of osteosarcoma cells
IF: 1.785Journal:Experimental and Therapeutic Medicine -
EMX2OS Delays Wilms’Tumor Progression via Targeting miR-654-3p
IF: 1.256Journal:ANNALS OF CLINICAL AND LABORATORY SCIENCEPMID:35181613
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |