RIPA Lysis Buffer (Strong)
中文名称:RIPA裂解液 (强)
Price:
Size:
100mL
50mL
20mL
- 应用性: WB
产品简介 | RIPA裂解液是一种传统的细胞组织快速裂解液,作为Western Blot试验中从动物组织或细胞中提取蛋白的首选裂解液。 |
保存条件 | -20°C冻存,保质期12个月。 |
注意事项 | 1. 裂解样品的所有步骤都需在冰上或4°C进行。 2. PMSF和原矾酸钠在使用前添加,每1 mL裂解液中加入10 μL PMSF和10 μL Na3VO4,使终浓度为1 mM。如发现RIPA有沉淀,请放置室温或温水浴进行溶解。 3. RIPA裂解液的裂解产物中可能会出现一团透明胶状物,属正常现象。该透明胶状物为含有基因组DNA等的复合物。用移液器反复吹打样本50次左右,可以有效降低样本样本粘度,离心后取上清进行后续试验。 4. 用RIPA裂解的得到的蛋白样品,因含有高浓度的去垢剂,不能用Bradford法测定样品的蛋白浓度,建议使用BCA法测定蛋白浓度。 5. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 |
-
Carvedilol activates nuclear factor E2-related factor 2/ antioxidant response element pathway to inhibit oxidative stress and apoptosis of retinal pigment epithelial cells induced by high glucose
IF: 3.269 -
CircRNA ZNF609 promotes the growth and metastasis of thyroid cancer in vivo and in vitro by downregulating miR-514a-5p
IF: 3.269 -
Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats
IF: 3.251Journal:Life-Basel -
IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation
IF: 3.246 -
Circ_0007580 knockdown strengthens the radiosensitivity of non-small cell lung cancer via the miR-598-dependent regulation of THBS2
IF: 3.223Journal:Thoracic Cancer -
Quaking I-5 protein inhibits invasion and migration of kidney renal clear cell carcinoma via inhibiting epithelial-mesenchymal transition suppression through the regulation of microRNA 200c
IF: 3.15 -
Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway
IF: 3.093 -
Long non-coding RNA SNHG16 decreased SMAD4 to induce gemcitabine resistance in pancreatic cancer via EZH2-mediated epigenetic modification
IF: 2.996 -
Knockdown of CDCA5 suppresses malignant progression of breast cancer cells by regulating PDS5A
IF: 2.952 -
CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2
IF: 2.945
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |