25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit
中文名称：25羟基维生素D3(25-HVD3)酶联免疫吸附测定试剂盒
别称：
25-Hydroxycholecalciferol、Calcidiol
Price:
Size:
96T
48T
96T*5
- 反应性： Universal
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用25-HVD3抗原包被于酶标板上，实验时样品或标准品中的25-HVD3与包被的25-HVD3竞争生物素标记的抗25-HVD3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，25-HVD3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中25-HVD3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 2.5h |
| 反应性 | Universal |
| 检测方法 | Colormetric |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 50μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的25-HVD3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
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Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats
IF: 6.1 -
A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism
IF: 4.545 -
Lactobacillus rhamnosus GG Promotes Intestinal Vitamin D Absorption by Upregulating Vitamin D Transporters in Senile Osteoporosis
IF: 4.333 -
Hydrophilic and Insoluble Electrospun Cellulose Acetate Fiber-Based Biosensing Platform for 25-Hydroxy Vitamin-D3 Detection
IF: 4.089Journal：ACS Applied Polymer Materials -
Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B
IF: 3.932 -
Postnatal Vitamin D Intake Modulates Hippocampal Learning and Memory in Adult Mice
IF: 3.877Journal：Frontiers in Neuroscience -
Chronic vitamin D3 supplementation alleviates cognition impairment via inhibition of oxidative stress regulated by PI3K/AKT/Nrf2 in APP/PS1 transgenic mice
IF: 3.046 -
Brazil nut intake increases circulating miR-454-3p and miR-584-5p in obese women
IF: 2.627Journal：NUTRITION RESEARCH -
Bacillus Calmette-Guérin (BCG) stimulates changes in dendritic cell surface marker expression in vitamin D-deficient mice:
IF: 1.287Journal：JOURNAL OF INTERNATIONAL MEDICAL RESEARCH -
Determinants for low bone mineral density in pre-school children: a matched case-control study in Wuhan, China
IF: 1.239Journal：JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM
