AA(Arachidonic Acid) ELISA Kit
中文名称:花生四烯酸(AA)酶联免疫吸附测定试剂盒
别称:
ARA
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用AA抗原包被于酶标板上,实验时样品或标准品中的AA与包被的AA竞争生物素标记的抗AA单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,AA浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中AA的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的AA,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation
IF: 17.521 -
Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling
IF: 6.684Journal:Frontiers in Cell and Developmental Biology -
COX-2/PGE2 upregulation contributes to the chromosome 17p-deleted lymphoma
IF: 6.524Journal:Oncogenesis -
Systems biology identifies cytosolic PLA2 as a target in vascular calcification treatment
IF: 6.014Journal:JCI Insight -
Dapagliflozin Reduces Apoptosis of Diabetic Retina and Human Retinal Microvascular Endothelial Cells Through ERK1/2/cPLA2/AA/ROS Pathway Independent of Hypoglycemic.
IF: 5.811Journal:Frontiers in Pharmacology -
The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats
IF: 5.555Journal:Frontiers in Endocrinology -
TRIB2 desensitizes ferroptosis via βTrCP-mediated TFRC ubiquitiantion in liver cancer cells
IF: 5.241 -
Role of T lymphocytes and papain enzymatic activity in the protection induced by the cysteine protease against Schistosoma mansoni in mice
IF: 5.045 -
Is arachidonic acid an endoschistosomicide?
IF: 4.327Journal:Journal of Advanced Research -
Specific Antibodies and Arachidonic Acid Mediate the Protection Induced by the Schistosoma mansoni Cysteine Peptidase-Based Vaccine in Mice
IF: 4.086