Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
中文名称：碱性磷酸酶(ALP)比色法测试盒(对硝基苯磷酸二钠法)
Price:
Size:
96T(79 samples)
48T(31 samples)
- 样本类型： 血清（浆）;组织;细胞
- 检测仪器及波长： 酶标仪(400-415 nm，最佳检测波长405 nm)
- 研究领域： 肝肾功能
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
在碱性条件下，碱性磷酸酶催化对硝基苯磷酸二钠水解，生成对硝基苯酚和磷酸。对硝基苯酚在强碱性条件下呈亮黄色，在405 nm处有强烈的吸收峰。因此可以测定405 nm 处的吸收值来计算产物的生成量，从而计算出酶活。
|
| 灵敏度 | 0.27 U/L |
| 检测范围 | 0.27-50.8 U/L |
| 精密度 | 批间差：4.6% | 批内差：1.2% |
| 检测时间 | 30 min |
| 检测仪器及波长 | 酶标仪(400-415 nm，最佳检测波长405 nm) |
| 保存条件 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
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| E-BC-K094-M | Acid Phosphatase (ACP) Activity Assay Kit | 96T(80 samples) , 48T(32 samples) |
| E-BC-K196-M | 5’-Nucleotidase (5'-NT) Activity Assay Kit | 96T(80 samples) , 48T(32 samples) |
