cAMP(Cyclic adenosine monophosphate) ELISA Kit
中文名称：环磷酸腺苷(cAMP)酶联免疫吸附测定试剂盒
别称：
Cyclic adenosine monophosphate、CAMP、Cyclic AMP、3'-5'-cyclic adenosine monophosphate
Price:
Size:
96T
48T
96T*5
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 2.5h |
| 反应性 | Universal |
| 检测方法 | Colormetric |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50μL |
| 样本类型 | 血浆，细胞上清，细胞提取液体，组织匀浆等其他生物体液 |
| 特异性 | 可检测样本中的cAMP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
-
The gut microbe Bacteroides fragilis ameliorates renal fibrosis in mice
IF: 17.694 -
Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice
IF: 8.469 -
Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1β-Induced Endothelium Injury
IF: 7.675Journal：Antioxidants -
Forskolin, an Adenylcyclase/cAMP/CREB Signaling Activator Restoring Myelin-Associated Oligodendrocyte Destruction in Experimental Ethidium Bromide Model of Multiple Sclerosis
IF: 7.666 -
Phosphodiesterase 4B activation exacerbates pulmonary hypertension induced by intermittent hypoxia by regulating mitochondrial injury and cAMP/PKA/p-CREB/PGC-1α signaling
IF: 7.419Journal：BIOMEDICINE & PHARMACOTHERAPY -
Site-specific N-terminal PEGylation-based controlled release of biotherapeutics: An application for GLP-1 delivery to improve pharmacokinetics and prolong hypoglycemic effects
IF: 7.088 -
Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway
IF: 7.076 -
ONO-1301 loaded nanocomposite scaffolds modulate cAMP mediated signaling and induce new bone formation in critical sized bone defect
IF: 6.843Journal：Biomaterials Science -
A Novel Furocoumarin Derivative, 5-((diethylamino)me-13 thyl)-3-phenyl-7H-furo [3,2-g] chromen-7-one Upregulates Melanin Synthesis via the Activation of cAMP/PKA and MAPKs Signal Pathway: In Vitro and In Vivo Study
IF: 6.208Journal：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -
Conjunctival Fluid Secretion Impairment via CaCC-CFTR Dysfunction Is the Key Mechanism in Environmental Dry Eye
IF: 6.208Journal：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Q1:I saw that when the concentration of cAMP was measured by competitive ELISA kit in the literature, 0.1M HCl-lysed cells were used. Can we use it?
We recommend that you refer to the cell sample treatment in the instructions instead of using 0.1M HCl for cell lysis. In theory, 0.1M hydrochloric acid can only change the permeability of cells and cannot lyse cells. At the same time, the introduction of strong acids will lead to the degeneration of proteins, which will affect the binding of antigens and antibodies, and change the pH value of the ELISA experimental system, and ultimately affect the experimental results.
