Cell Total Iron Colorimetric Assay Kit
中文名称:细胞总铁比色法测试盒
Price:
Size:
96T(80 samples)
48T(32 samples)
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(590 - 600 nm,最佳检测波长为593 nm)
- 研究领域: 无机盐离子,铁死亡
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
在还原剂的作用下,样本中的铁离子被还原为亚铁离子,亚铁离子与探针结合,生成的物质在593 nm波长处有强吸收峰,在一定范围内其光密度值与铁离子浓度成线性相关。
|
灵敏度 | 0.4 μmol/L |
检测范围 | 0.4-50 μmol/L |
精密度 | 批间差:1.5 | 批内差:1.3 |
检测时间 | 80min |
检测仪器及波长 | 酶标仪(590 - 600 nm,最佳检测波长为593 nm) |
保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:How is the cell sample broken?
The kit used the matching cell lysis buffer to process the cell samples. After adding the lysis buffer, it only needs to stand on the ice box for 10 minutes, then the supernatant can be centrifuged to be tested, which effectively improves the cell crushing efficiency.
Q2:Can the final result be calculated not by cell number but by protein concentration?
The reagent 1 used in sample preparation will affect the determination of protein concentration, so it can not be calculated by protein concentration, but by cell number.