F-TESTO(Free Testosterone) ELISA Kit
中文名称:游离睾酮(F-TESTO)酶联免疫吸附测定试剂盒
F-TESTO
Price:
- 反应性: Universal
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用F-TESTO抗原包被于酶标板上,实验时样品或标准品中的F-TESTO与包被的F-TESTO竞争生物素标记的抗F-TESTO单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,F-TESTO浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中F-TESTO的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的F-TESTO,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats
IF: 6.291 -
Impact of bariatric surgery on androgen profile and ovarian volume in obese polycystic ovary syndrome patients with infertility
IF: 4.219 -
Ameliorative Effect of VE, IGF-I, and hCG on the Fluoride-Induced Testosterone Release Suppression in Mice Leydig Cells
IF: 3.738Journal:BIOLOGICAL TRACE ELEMENT RESEARCH -
The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice
IF: 3.412 -
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes
IF: 3.227Journal:REPRODUCTIVE TOXICOLOGY -
Competitive endogenous RNA (ceRNA) regulation network of lncRNA-miRNA-mRNA during the process of the nickel-induced steroidogenesis disturbance in rat Leydig cells
IF: 3.067Journal:TOXICOLOGY IN VITRO -
Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy
IF: 2.734Journal:Andrology -
Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats
IF: 2.427Journal:Andrology -
Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes
IF: 2.376 -
Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice
IF: 1.723
Q1:I want to measure the content of testosterone in the supernatant of cell culture. Does the culture medium containing phenol red affect the detection of testosterone?
The medium containing phenol red may have an effect on the detection of testosterone. Phenol red is used in the medium as an indicator of PH: red when neutral, yellow when acidic, and purple when alkaline. Studies have shown that phenol red can mimic the effects of sterol hormones (especially estrogen). To avoid sterol reactions, cultured cells, especially mammalian cells, are cultured without phenol red. Introduce a tip: when the medium is packaged, leave a tube with phenol red and put it in the incubator to balance (you can see the change in PH value, and you can see whether the medium is polluted). Put in the incubator before culture for balanced use (can avoid the use of phenol red during culture).