F-TESTO(Free Testosterone) ELISA Kit
中文名称:游离睾酮(F-TESTO)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用F-TESTO抗原包被于酶标板上,实验时样品或标准品中的F-TESTO与包被的F-TESTO竞争生物素标记的抗F-TESTO单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,F-TESTO浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中F-TESTO的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其它相关生物液体 |
特异性 | 可检测样本中的F-TESTO,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats
IF: 6.291 -
Impact of bariatric surgery on androgen profile and ovarian volume in obese polycystic ovary syndrome patients with infertility
IF: 4.219 -
Ameliorative Effect of VE, IGF-I, and hCG on the Fluoride-Induced Testosterone Release Suppression in Mice Leydig Cells
IF: 3.738Journal:BIOLOGICAL TRACE ELEMENT RESEARCH -
The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice
IF: 3.412 -
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes
IF: 3.227Journal:REPRODUCTIVE TOXICOLOGY -
Competitive endogenous RNA (ceRNA) regulation network of lncRNA-miRNA-mRNA during the process of the nickel-induced steroidogenesis disturbance in rat Leydig cells
IF: 3.067Journal:TOXICOLOGY IN VITRO -
Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy
IF: 2.734Journal:Andrology -
Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats
IF: 2.427Journal:Andrology -
Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes
IF: 2.376 -
Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice
IF: 1.723