FA/VB9(Folic Acid/Vitamin B9) ELISA Kit
中文名称:叶酸/维生素B9(FA/VB9)酶联免疫吸附测定试剂盒
别称:
FA、Pteroyl-L-glutamic acid、Vitamin B9、Vitamin Bc、Vitamin M、Folacin、Pteroyl-L-glutamate
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用FA/VB9抗原包被于酶标板上,实验时样品或标准品中的FA/VB9与包被的FA/VB9竞争生物素标记的抗FA/VB9单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,FA/VB9浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中FA/VB9的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的FA/VB9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
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Mitochondrial DNA and Epigenetics: Investigating Interactions with the One-Carbon Metabolism in Obesity
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Folic Acid Alleviates High Glucose and Fat-Induced Pyroptosis via Inhibition of the Hippo Signal Pathway on H9C2 Cells
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Folate Metabolism Regulates Oligodendrocyte Survival and Differentiation by Modulating AMPKα Activity
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Comparison of Associations between One-Carbon Metabolism, Lipid Metabolism, and Fatty Liver Markers in Normal-Weight and Overweight People Aged 20–40 Years
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Low folate intake and serum levels are associated with higher body mass index and abdominal fat accumulation: a case control study.
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Comparison of serum B12, folate and homocysteine concentrations in children with autism spectrum disorder or attention deficit hyperactivity disorder and healthy controls
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Klotho, vitamin D and homocysteine levels during acute episode and remission periods in schizophrenia patients
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Dietary vitamin B12 requirement and its effect on non-specific immunity and disease resistance in juvenile Chinese mitten crab Eriocheir sinensis
IF: 1.828Journal:AQUACULTURE