GnRH(Gonadotropin Releasing Hormone) ELISA Kit
中文名称:促性腺激素释放激素(GnRH)酶联免疫吸附测定试剂盒
别称:
GNRH1、GRH、HH12、LHRH、LNRH、Luliberin
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用GnRH抗原包被于酶标板上,实验时样品或标准品中的GnRH与包被的GnRH竞争生物素标记的抗GnRH单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,GnRH浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中GnRH的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的GnRH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The epigenetic role of HTR1A antagonist in facilitating GnRH expression for pubertal initiation control
IF: 8.886Journal:Molecular Therapy-Nucleic Acids -
Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats
IF: 7.963 -
Perfluorooctanoic acid exposure impact a trade-off between self-maintenance and reproduction in lizards (Eremias argus) in a gender-dependent manner
IF: 6.793Journal:ENVIRONMENTAL POLLUTION -
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 6.6 -
Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway
IF: 6.53 -
Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway
IF: 6.4 -
Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice
IF: 6.291 -
In utero exposure to DBP stimulates release of GnRH by increasing the secretion of PGE2 in the astrocytes of the hypothalamus in the offspring mice
IF: 6.291 -
Enrichment of imidacloprid and its metabolites in lizards and its toxic effects on gonads
IF: 5.714 -
Accumulation and toxicity of thiamethoxam and its metabolite clothianidin to the gonads of Eremias argus
IF: 5.589