Human AP13(Apelin 13) ELISA Kit
中文名称:人Apelin 13蛋白(AP13)酶联免疫吸附测定试剂盒
别称:
AP13
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 125-8000 pg/mL
- 灵敏度: 75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人AP13抗原包被于酶标板上,实验时样品或标准品中的人AP13与包被的人AP13竞争生物素标记的抗人AP13单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,人AP13浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中人AP13的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 125-8000 pg/mL |
灵敏度 | 75 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人AP13,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The Association Between Serum Apelin-13 and the Prognosis of Acute Ischemic Stroke
IF: 5.78Journal:Translational Stroke Research -
Serum apelin-13 and risk of death following severe traumatic brain injury
IF: 3.786 -
Evaluation of Apelin-13 levels in patients with diabetic nephropathy
IF: 2.266 -
Exploratory study to evaluate plasma vasopressin and apelin‐13 levels in children with attention‐deficit hyperactivity disorder
IF: 2.025Journal:PSYCHIATRY AND CLINICAL NEUROSCIENCES -
Apelin-13: A Promising Biomarker for Age-Related Macular Degeneration?
IF: 1.926Journal:OPHTHALMOLOGICA -
Evaluation of serum apelin‐13 and apelin‐36 concentrations in preeclamptic pregnancies
IF: 1.122Journal:JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH -
The investigation effect of weight loss on serum vaspin, apelin-13, and obestatin levels in obese individual
IF: 0.373Journal:Turkish Journal of Biochemistry-Turk Biyokimya Dergisi -
Peculiarities Of Changes Of Apelin-13 Concentration In Patients With Essential Hypertension And Extrasystole
Journal:Arterial Hypertension