Human APLN(Apelin) ELISA Kit
中文名称:人Apelin蛋白(APLN)酶联免疫吸附测定试剂盒
别称:
APEL、XNPEP2
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 62.5-4000 pg/mL
- 灵敏度: 37.5 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人APLN抗原包被于酶标板上,实验时样品或标准品中的人APLN与包被的人APLN竞争生物素标记的抗人APLN单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,人APLN浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中人APLN的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 62.5-4000 pg/mL |
灵敏度 | 37.5 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人APLN,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Elabela Attenuates the TGF-β1-Induced Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells in Patients Receiving Peritoneal Dialysis
IF: 5.988 -
The Effect of SGLT2 Inhibitor Dapagliflozin on Serum Levels of Apelin in T2DM Patients with Heart Failure
IF: 4.757 -
Interplay between Myokine Profile and Glycemic Control in Type 2 Diabetes Mellitus Patients with Heart Failure
IF: 3.992Journal:Diagnostics -
Discriminative Utility of Apelin-to-NT-Pro-Brain Natriuretic Peptide Ratio for Heart Failure with Preserved Ejection Fraction among Type 2 Diabetes Mellitus Patients
IF: 3.948Journal:Journal of Cardiovascular Development and Disease -
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
IF: 3.239 -
Association of serum vaspin, apelin, and visfatin levels and stroke risk in a Chinese case-control study
IF: 1.889 -
Evaluation of Fibrosis Markers: Apelin and Transforming Growth Factor‐β1 in Autosomal Dominant Polycystic Kidney Disease Patients
IF: 1.477Journal:THERAPEUTIC APHERESIS AND DIALYSIS -
Serum Apelin Levels and Ascending Aortic Aneurysm in Adults
IF: 0.561Journal:Indian Journal of Surgery -
Association of Apelin gene rs3115757 variation and expression with metabolic syndrome among female students in King Abdulaziz University
Journal:Gene Reports