Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit
中文名称：线粒体呼吸链复合物I(NADH-辅酶Q还原酶)比色法测试盒
Price:
- 样本类型： 动物组织
- 检测仪器及波长： 酶标仪(340 nm)
- 研究领域： 线粒体系列,铜死亡
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
线粒体呼吸链复合物I催化NADH与泛醌底物反应，生成NAD+与还原型泛醌，检测340 nm处NADH吸光度下降速率可反应其活力，特异性抑制剂鱼藤酮可抑制该酶，总酶活减去抑制剂抑制后的酶活可反映其特异性酶活。
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| 灵敏度 | 15.58 U/L |
| 检测范围 | 15.58-224.2 U/L |
| 精密度 | 批间差：8.4 | 批内差：0.8 |
| 检测时间 | 15 min |
| 检测仪器及波长 | 酶标仪(340 nm) |
| 保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
| E-BC-K150-M | Mitochondrial Complex II Activity Assay Kit | 96T(94 samples) , 48T(46 samples) |
| E-BC-K151-M | Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome c Reductase) Activity Assay Kit | 96T(47 samples) , 48T(23 samples) |
| E-BC-K152-M | Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit | 96T(94 samples) , 48T(46 samples) |
Q1:If the tissue sample is less than 0.1g, can it be used for experiments?How should the test dose be adjusted during sample preparation?
If the tissue weight is less than 0.1g, it can be reduced according to the proportion of tissue weight and homogenate volume as described in the manual. It is necessary to ensure that the homogenated volume of the final sample is sufficient for mitochondrial complex I detection and protein concentration detection.
Q2:For tissue samples, must the protein concentration be tested?
Tissue samples need to be homogenized, and the protein concentration released varies with the degree of homogenization. Therefore, by detecting the protein content in the sample, the difference in the homogenization process of the sample can be corrected to ensure the accuracy of the results. Therefore, tissue samples need to detect the protein concentration.
Q3:In the manual, it is recommended to test no more than 8 samples at a time. Does this refer to the number of samples or the number of sample wells? For example, if I need to do 2 duplicate wells for each sample, is it recommended that the number of tests be no more than 8 or 4?
Because the reaction rate of this kit is relatively fast and the control of time is very accurate, it is recommended that the number of sample wells should not exceed 8 at a time to ensure the accuracy of the measurement results. If each sample does 2 double wells, the number of samples tested at one time should not exceed 4.
Q4:For tissue samples. Must it be fresh samples? Can frozen samples be tested?
It is recommended to use fresh samples as far as possible. If the samples are frozen, the results may be low or undetectable.The longer the frozen storage time, the greater the impact on the results. Generally, the sample should not be stored at -80℃ for more than one month. If the frozen storage time is more than one month, it is not recommended to use.
