NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
中文名称：去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上，实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，NA/NE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中NA/NE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的NA/NE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Manipulation of Glutamatergic Neuronal Activity in the Primary Motor Cortex Regulates Cardiac Function in Normal and Myocardial Infarction Mice
IF: 15.100 -
β-Receptor blocker enhances the anabolic effect of PTH after osteoporotic fracture
IF: 12.700 -
Beta2 adrenergic receptor–mediated abnormal myelopoiesis drives neuroinflammation in aged patients with traumatic brain injury
IF: 11.700Journal： Science Advances (2024)Reactivity：Human,MouseSample Type：serum -
Lysophosphatidic acid contributes to myocardial ischemia/reperfusion injury by activating TRPV1 in spinal cord
IF: 9.500 -
Circadian light/dark cycle reversal exacerbates the progression of chronic kidney disease in mice
IF: 8.300 -
Prevalence of perturbed gut microbiota in pathophysiology of arsenic-induced anxiety- and depression-like behaviour in mice
IF: 8.100Journal： CHEMOSPHERE (2024)Reactivity：MouseSample Type：brain tissue -
Remodelling the inguinal adipose sensory system to switch on the furnace: Electroacupuncture stimulation induces brown adipose thermogenesis
IF: 5.800Journal： DIABETES OBESITY & METABOLISM (2024)PMID：38229447Reactivity：MouseSample Type：Brown adipose tissue (BAT) -
A Combination of Exercise and Yogurt Intake Protects Mice against Obesity by Synergistic Promotion of Adipose Browning
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：Plasma -
Neuregulin4-ErbB4 signalling pathway is driven by electroacupuncture stimulation to remodel brown adipose tissue innervation
IF: 5.400Journal： DIABETES OBESITY & METABOLISM (2024)Reactivity：MouseSample Type：interscapular brown adipose tissue (iBAT) -
Taurocholic acid ameliorates hypertension through activation of TGR5 in hypothalamic paraventricular nucleus
IF: 5.100
实验操作视频
