PGE2(Prostaglandin E2) ELISA Kit
中文名称：前列腺素E2(PGE2)酶联免疫吸附测定试剂盒
别称：
PG-E2、Dinoprostone
Price:
Size:
96T
48T
96T*5
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用PGE2抗原包被于酶标板上，实验时样品或标准品中的PGE2与包被的PGE2竞争生物素标记的抗PGE2单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，PGE2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中PGE2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 2.5h |
| 反应性 | Universal |
| 检测方法 | Colormetric |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的PGE2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
-
Cancer cell-intrinsic XBP1 drives immunosuppressive reprogramming of intratumoral myeloid cells by promoting cholesterol production
IF: 31.373 -
Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism
IF: 10.723 -
ATP release drives heightened immune responses associated with hypertension
IF: 10.551Journal：Science Immunology -
Melanoma-specific bcl-2 promotes a protumoral M2-like phenotype by tumor-associated macrophages
IF: 10.252 -
Diet-Induced Hyperhomocysteinaemia Increases Intestinal Inflammation in an Animal Model of Colitis
IF: 9.071Journal：Journal of Crohns & Colitis -
Polydopamine-modified decellularized intestinal scaffolds loaded with adipose-derived stem cells promote intestinal regeneration
IF: 7.571Journal：Journal of Materials Chemistry B -
Treatment With Endothelin-A Receptor Antagonist BQ123 Attenuates Acute Inflammation in Mice Through T-Cell-Dependent Polymorphonuclear Myeloid-Derived Suppressor Cell Activation
IF: 7.561 -
Xiaojianzhong decoction attenuates gastric mucosal injury by activating the p62/Keap1/Nrf2 signaling pathway to inhibit ferroptosis
IF: 7.419 -
Multiple 'omics'-analysis reveals the role of prostaglandin E2 in Hirschsprung's disease
IF: 7.376 -
Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescence
IF: 7.08Journal：eLife
Q1:What is the difference between PGD2 and PGE2?
These two belong to different types of prostaglandins. Prostaglandin E2 (PGE2) is the most abundant prostaglandin and is produced by the action of prostaglandin E synthetase on prostaglandin H2(PGH2). Prostaglandin D2(PGD2) is another prostaglandin produced by hematopoietic and lipoprotein prostaglandin D synthase (hPGDS and lPGDS) acting on prostaglandin H2(PGH2). In addition, the two have different receptors and different areas of study. PGE2: Mainly studies gastrointestinal smooth muscle PGD2: Studies allergic diseases such as asthma
