PGI2(Prostacyclin) ELISA Kit
中文名称：前列环素(PGI2)酶联免疫吸附测定试剂盒
别称：
prostaglandin I2、PGI2
Price:
Size:
96T
48T
96T*5
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用PGI2抗原包被于酶标板上，实验时样品或标准品中的PGI2与包被的PGI2竞争生物素标记的抗PGI2单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，PGI2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中PGI2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96T
/ 48T
/ 96T*5
|
| 反应时间 | 2.5h |
| 反应性 | Universal |
| 检测方法 | Colormetric |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的PGI2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 数据处理 |
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Ginsenoside Rg1-Notoginsenoside R1-Protocatechuic Aldehyde Reduces Atherosclerosis and Attenuates Low-Shear Stress-Induced Vascular Endothelial Cell Dysfunction
IF: 5.811 -
Anti-platelet aggregation of Panax notoginseng triol saponins by regulating GP1BA for ischemic stroke therapy
IF: 5.455 -
Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia
IF: 4.38 -
Microsomal Prostaglandin E2 Synthase-1 Deletion Attenuates Isoproterenol-Induced Myocardial Fibrosis in Mice
IF: 3.561Journal：JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS -
Dexamethasone-Loaded Thermosensitive Hydrogel Suppresses Inflammation and Pain in Collagen-Induced Arthritis Rats
IF: 3.216 -
Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7
IF: 3.009Journal：Contrast Media & Molecular Imaging -
Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats
IF: 2.575 -
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats
IF: 2.162Journal：Food & Nutrition Research -
Fibronectin modified TiO2 nanotubes modulate endothelial cell behavior:
IF: 2.082Journal：JOURNAL OF BIOMATERIALS APPLICATIONS
Q1:PGI2(Prostacycline)ELISA kit(E-EL-0022), for the determination of PGI2 protein, or its metabolite 6keto-PGFla(because PGI2 has a short half-life).
PGI2 is extremely unstable, with a half-life of 14.5 min in an aqueous solution with a pH of 7.48. In a neutral medium, it can also be hydrolyzed to 6-ketone PGFla. The kit design is PGI2, but because PGI2 is relatively unstable in biological fluid samples, the antibody we designed also cross-reacts to its metabolite 6-keto PGFla, aiming to react the total amount of PGI2 by detecting the content of 6-keto PGFla and PGI2.
