QuicKey Human VEGF-A(Vascular Endothelial Growth Factor A) ELISA Kit
中文名称:QuicKey-人血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.04-10 ng/mL
- 灵敏度: 0.03 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,在实验时间节省至少1小时的同时,获得更加灵敏和精确的实验结果。Elabscience 自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测人血清、血浆中VEGF-A浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人VEGF-A抗体包被于酶标板上,实验时样品(或标准品)及生物素化的抗人VEGF-A抗体同时加入酶标板中,样本(或标准品)中的人VEGF-A会与包被抗体结合,同时抗人VEGF-A抗体与结合在包被抗体上的人VEGF-A结合,游离的成分被洗去。再加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.04-10 ng/mL |
灵敏度 | 0.03 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的人VEGF-A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
数据处理 |
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