QuicKey Pro Human Cortisol ELISA Kit
中文名称:QuicKey Pro-人皮质醇(Cortisol)酶联免疫吸附测定试剂盒
别称:
Cortisol
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 6.25-400 ng/mL
- 灵敏度: 2.87 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测人血清、血浆、唾液、尿液中Cortisol浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人Cortisol抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Cortisol单克隆抗体,样本(或标准品中)的Cortisol与包被抗原Cortisol竞争结合HRP标记的抗Cortisol单抗上的结合位点,形成包被抗原Cortisol-HRP标记的抗Cortisol单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cortisol浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Cortisol的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 1.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 6.25-400 ng/mL |
灵敏度 | 2.87 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆、唾液、尿液 |
特异性 | 可检测样本中的人Cortisol,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
数据处理 |
-
Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
IF: 9.988 -
Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide
IF: 7.476Journal:BIOSENSORS & BIOELECTRONICS -
Bifidobacterium breve CCFM1025 attenuates major depression disorder via regulating gut microbiome and tryptophan metabolism: A randomized clinical trial
IF: 7.217 -
Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test
IF: 5.068Journal:Journal of the International Society of Sports NutritionPMID:32907591 -
Human Physiological Responses to a Single Deep Helium-Oxygen Diving
IF: 4.566Journal:Frontiers in Physiology -
Mechanism of the Huangguoshu waterfall forest environment's promotive effect on human health in Guizhou, China.
IF: 3.616Journal:Annals of Translational Medicine -
Rutin protects against neuronal damage in vitro and ameliorates doxorubicin-induced memory deficits in vivo in Wistar rats
IF: 2.822Journal:Drug Design Development and Therapy -
The Effect of Copaiba Oil Odor on Anxiety Relief in Adults under Mental Workload: A Randomized Controlled Trial
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:35449818 -
An investigation into psychological stress and its determinants in Xinjiang desert oil workers
IF: 2.028Journal:MEDICINE -
Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery
IF: 0.545Journal:JOURNAL OF OBSTETRICS AND GYNAECOLOGY