Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
中文名称:大鼠血管紧张素Ⅱ(Ang-Ⅱ)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Ang-Ⅱ抗原包被于酶标板上,实验时样品或标准品中的大鼠Ang-Ⅱ与包被的大鼠Ang-Ⅱ竞争生物素标记的抗大鼠Ang-Ⅱ单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,大鼠Ang-Ⅱ浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中大鼠Ang-Ⅱ的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠Ang-Ⅱ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Simultaneous inhibition of neprilysin and activation of ACE2 prevented diabetic cardiomyopathy
IF: 2.761Journal:Pharmacological Reports -
Angiotensin aldosterone inhibitors improve survival and ameliorate kidney injury induced by sepsis through suppression of inflammation and apoptosis
IF: 2.748 -
The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy
IF: 2.73Journal:EUROPEAN JOURNAL OF PHARMACOLOGY -
Cardio-protective effects of a dioxidovanadium(V) complex in male sprague–dawley rats with streptozotocin-induced diabetes
IF: 2.479Journal:BIOMETALS -
Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats
IF: 2.479 -
Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin‑angiotensin system activity
IF: 2.447Journal:Experimental and Therapeutic Medicine -
Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system
IF: 2.447Journal:Experimental and Therapeutic Medicine -
The effect of eplerenone on the renin‐angiotensin‐aldosterone system of rats with thyroid dysfunction
IF: 2.39Journal:JOURNAL OF PHARMACY AND PHARMACOLOGY -
Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction
IF: 2.287Journal:International Journal of Endocrinology -
Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue
IF: 1.927