Rat β-CTx(Beta Crosslaps) ELISA Kit
中文名称:大鼠β-骨胶原交联(β-CTx)酶联免疫吸附测定试剂盒
别称:
b-CTx、BCTXI、BCTX-I、B-Cr、BCL、Type I Collagen C-Telopeptide-Related Fraction
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 125-8000 pg/mL
- 灵敏度: 75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠β-CTx抗原包被于酶标板上,实验时样品或标准品中的大鼠β-CTx与包被的大鼠β-CTx竞争生物素标记的抗大鼠β-CTx单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,大鼠β-CTx浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中大鼠β-CTx的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 125-8000 pg/mL |
灵敏度 | 75 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠β-CTx,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Chronic Sleep Deprivation Impaired Bone Formation in Growing Rats and Down-Regulated PI3K/AKT Signaling in Bone Tissues
IF: 5.346 -
Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B
IF: 3.932 -
Self-emulsified annatto tocotrienol improves bone histomorphometric parameters in a rat model of oestrogen deficiency through suppression of skeletal sclerostin level and RANKL/OPG ratio
IF: 3.738Journal:International Journal of Medical Sciences -
Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:35769158 -
Acteoside Derived from Cistanche Improves Glucocorticoid-Induced Osteoporosis by Activating PI3K/AKT/mTOR Pathway
IF: 1.972Journal:JOURNAL OF INVESTIGATIVE SURGERY -
Sheep bone collagen peptide ameliorates osteoporosis by regulating RANK/RANKL/OPG signal pathway
IF: 1.718Journal:Food Science and Technology