Rat β-CTx(Beta Crosslaps) ELISA Kit
中文名称:大鼠β-骨胶原交联(β-CTx)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 125-8000 pg/mL
- 灵敏度: 75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠β-CTx抗原包被于酶标板上,实验时样品或标准品中的大鼠β-CTx与包被的大鼠β-CTx竞争生物素标记的抗大鼠β-CTx单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,大鼠β-CTx浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中大鼠β-CTx的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 125-8000 pg/mL |
灵敏度 | 75 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠β-CTx,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Shengu granules ameliorate ovariectomy-induced osteoporosis by intestinal immune-skeletal signaling axis
IF: 5.200 -
Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production
IF: 3.900 -
Chronic Sleep Deprivation Impaired Bone Formation in Growing Rats and Down-Regulated PI3K/AKT Signaling in Bone Tissues
IF: 3.384 -
Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells
IF: 2.650Journal: Evidence-based Complementary and Alternative Medicine (2022)PMID:35769158Reactivity:RatSample Type:plasma -
Sheep bone collagen peptide ameliorates osteoporosis by regulating RANK/RANKL/OPG signal pathway
IF: 2.602 -
Acteoside Derived from Cistanche Improves Glucocorticoid-Induced Osteoporosis by Activating PI3K/AKT/mTOR Pathway
IF: 1.972 -
Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B
IF: 3.932 -
Self-emulsified annatto tocotrienol improves bone histomorphometric parameters in a rat model of oestrogen deficiency through suppression of skeletal sclerostin level and RANKL/OPG ratio
IF: 3.738Journal: International Journal of Medical Sciences (2021)PMID:34790038Reactivity:RatSample Type:serum