Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)
中文名称:活性氧(ROS)荧光法测试盒(绿色)
Price:
Size:
96T
- 样本类型: 组织;细胞
- 检测仪器及波长: 荧光酶标仪(Ex/Em=500 nm/525 nm)、流式细胞仪或荧光显微镜
- 研究领域: 氧化应激,代谢疾病,铁死亡
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
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- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
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- 46
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- 50
- 80
- 86
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- 100
- 480
x
所需盒子量:
检测原理 |
DCFH-DA(2,7-dichlorofuorescin diacetate)是一种可以自由穿过细胞膜的荧光探针,自身没有荧光,进入细胞内后,可以被细胞内的酯酶水解生成DCFH(dichlorofluorescin)。在活性氧存在时DCFH被氧化为不能透过细胞膜的强绿色荧光物质DCF(dichlorofluorescein),其荧光在激发波长502nm,发射波长530nm附近有最大波峰,强度与细胞内活性氧水平成正比。
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指标简称 | ROS |
检测时间 | 60 min |
检测仪器及波长 | 荧光酶标仪(Ex/Em=500 nm/525 nm)、流式细胞仪或荧光显微镜 |
保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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