Recombinant Human IGFBP-7 protein (His tag)
Price:
- 表达系统： HEK293 Cells
- 蛋白编码： Q16270
| 别称 |
IGF-binding protein 7;IGFBP-rP1;IGFBP7;Insulin-like growth factor-binding protein 7;MAC25 protein;TAF;Tumor-derived adhesion factor
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| 表达系统 |
HEK293 Cells
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| 序列 |
Met1-Leu282
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| 蛋白编码 |
Q16270
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| 种属 |
Human
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| 计算分子量 |
30.9 kDa
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| 表观分子量 |
32 kDa
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| 标签 |
C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
Insulin-like growth factor-binding protein 7(IGFBP-7) is a secreted glycosylated protein that contains three protein domain modules. IGFBP7 contains an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. Human and mouse IGFBP7 are highly homologous and share 94% aa sequence identity. It is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. It binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP7 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. It supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis.
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Q1:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q2:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q5:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q6:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q7:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
实验操作视频
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