Recombinant Human PAFAH1B2/PAFAHB Protein (His Tag)
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- 表达系统: E.coli
- 蛋白编码: P68402
别称 |
PAF Acetylhydrolase 30 kDa Subunit;PAF-AH 30 kDa Subunit;PAF-AH Subunit Beta;PAFAH Subunit Beta;PAFAH1B2;PAFAHB;Platelet-Activating Factor Acetylhydrolase IB Subunit Beta
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表达系统 |
E.coli
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序列 |
Ser2-Ala229
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蛋白编码 |
P68402
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种属 |
Human
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计算分子量 |
26.6 kDa
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表观分子量 |
31 kDa
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标签 |
C-His
|
生物活性 |
Not validated for activity
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
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复溶方法 |
Not Applicable
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背景 |
Platelet-Activating Factor Acetylhydrolase IB Subunit β (PAFAHB) is a cytoplasmic hydrolase. PAFAHB is a member of the GDSL lipolytic enzyme family. It also belongs to Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma), PAFAHB is a catalytic subunit. PAFAHB inactivates PAF by removing the acetyl group at the sn-2 position.
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Q1:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q2:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q3:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q4:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q5:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q6:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q7:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
实验操作视频
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