Recombinant Mouse ALPL protein(His tag)
Price:
Size:
100μg
20μg
- 表达系统: HEK293 Cells
- 蛋白编码: P09242
别称 |
AP-TNAP;TNAP;TNSALP;Alkaline phosphatase 2;Alkaline phosphatase liver/bone/kidney isozyme;Phosphoamidase;Phosphocreatine phosphatase
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表达系统 |
HEK293 Cells
|
序列 |
Met1-Ser502
|
蛋白编码 |
P09242
|
种属 |
Mouse
|
计算分子量 |
52.0 kDa
|
表观分子量 |
70 kDa
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标签 |
C-His
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纯度 |
> 80 % as determined by reducing SDS-PAGE.
|
内毒素 |
Please contact us for more information.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
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实验操作视频
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