Recombinant Rat CCl2 protein (His tag)
Price:
- 表达系统: HEK293 Cells
- 蛋白编码: P14844
别称 |
C-C motif chemokine 2;Ccl2;Immediate-early serum-responsive protein JE;MCP-1;Mcp1;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;Scya2;Small-inducible cytokine A2
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表达系统 |
HEK293 Cells
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序列 |
Met1-Asn148
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蛋白编码 |
P14844
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种属 |
Rat
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计算分子量 |
13.6 kDa
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表观分子量 |
40 kDa
|
标签 |
C-His
|
生物活性 |
Not validated for activity
|
纯度 |
> 95% as determined by reducing SDS-PAGE.
|
内毒素 |
Please contact us for more information.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP)-1 and small inducible cytokine A2 (SCYA2)), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 are implicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention.
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Q1:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q2:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q3:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q4:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q5:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q6:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q7:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
实验操作视频
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